Title: SsHKT1;1 is coordinated with SsSOS1 and SsNHX1 to regulate Na⁺ homeostasis in Suaeda salsa under saline conditions

Authors: Wen-Ying Wang, Ya-Qi Liu, Hui-Rong Duan, Xiu-Xia Yin, Yan-Nong Cui, Wei-Wei Chai, Xin Song, Timothy J. Flowers, Suo-Min Wang*

Journal: Plant and Soil (农林科学一区)

DOI: https://doi.org/10.1007/s11104-020-04463-x

IF: 3.299

Abstract: Background Under saline conditions, Suaeda salsa, as a typical halophyte, accumulates large amounts of Na⁺ in its leaves during optimal growth. Key transporters involved in Na⁺ accumulation in plants are HKT-type protein, the plasma membrane Na⁺/H⁺ transporter SOS1, and the tonoplast Na⁺/H⁺ antiporter NHX1. In this study, the function of SsHKT1;1 and its coordinate expression with SsSOS1 and SsNHX1 to regulate Na⁺ homeostasis in S. salsa was investigated.

Results We showed, by yeast complementation assays, that SsHKT1;1 encoded a Na⁺-selective transporter, which located to the plasma membrane and was preferentially expressed within the stele, and was particularly abundant in xylem parenchyma and pericycle cells. When compared with a treatment of 25 mM NaCl, 150 mM NaCl greatly decreased the transcripts of SsHKT1;1, but maintained a relatively constant level of the expression of SsSOS1 in roots. Consequently, the synergistic effect of SsHKT1;1 and SsSOS1 would result in greater Na⁺ loading into the xylem under 150 mM NaCl than 25 mM NaCl. In leaves, 150 mM NaCl up-regulated the abundance of SsNHX1 compared with levels in 25 mM NaCl. This enabled the permanent sequestering of Na⁺ into leaf vacuoles.

Conclusions Overall, SsHKT1;1 functioned in reducing Na⁺ retrieval from the root xylem, and played an important role in coordinating with SsSOS1 and SsNHX1 to maintain Na⁺ accumulation in S. salsa under saline conditions.

链接: https://link.springer.com/article/10.1007/s11104-020-04463-x